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Glycine

Human RCT Supplementssleep Last reviewed 2026-09-28

Summary

A nonessential amino acid. Small Japanese RCTs using 3 g before bed report improved subjective sleep quality and reduced next-day sleepiness, including one trial with polysomnographic measurements.

Mechanism

Glycine is a nonessential amino acid and an inhibitory neurotransmitter. The proposed mechanism is action on NMDA receptors in the suprachiasmatic nucleus, the brain's circadian pacemaker, promoting peripheral vasodilation and a drop in core body temperature, a physiological change that normally accompanies sleep onset.

Reported effects

Ratings are relative to peers in the same category, not absolute claims.

Subjective sleep quality★★★☆☆Three small RCTs using 3 g before bed report better subjective sleep vs placebo.
Next-day sleepiness and fatigue★★☆☆☆Reduced morning sleepiness and fatigue reported in small crossover trials.

Studied dosing: research context

Published trials used 3 g taken 30-60 minutes before bedtime, for 2-4 nights in most studies. These are doses used in research, not guidance.

Research context only. This is not a recommendation.

Safety

No serious adverse events were observed at 3 g in published trials. A small safety study at 9 g reported only mild, transient digestive symptoms (soft stool, stomach discomfort) and no carryover daytime sleepiness. Data in pregnancy are limited.

Limitations

Trials are very small (mostly under 20 participants) and short, and all come from the same research group with ties to a glycine manufacturer. Independent replication in larger, longer trials is missing, and effects on objective sleep architecture are minimal.

References

  1. Yamadera et al., Sleep Biol Rhythms (2007) - glycine ingestion and subjective sleep quality with polysomnography
  2. Inagawa et al., Sleep Biol Rhythms (2006) - subjective effects of glycine ingestion before bedtime
  3. Bannai et al., Front Neurol (2012) - glycine and daytime performance in sleep-restricted volunteers